Target-Screening of Antibiotic Resistance Genes in Water Environments in Viet Nam and Japan

Day: Friday, August 21, 2020

Time: 20:00 - 21:30

Venue: Webinar Map

Seminar

Abstract – Screening for key antibiotic resistance genes (ARGs) and their surrogate markers in water environments is an indispensable tool for the efficient monitoring and management of environmental health. We applied SmartChip Real-Time PCR technology to characterize ARGs in wastewater in Vietnam and Japan. 195 out of 384 target genes were detected from domestic wastewater in Vietnam. Although 156 out of 195 genes were commonly detected in domestic wastewater in Japan, the relative abundances of 109 genes including mobile colistin resistance (mcr-1) were higher in the wastewater in Vietnam than in Japan. Surveillance of mcr-1 revealed that this gene was prevalent in water environments and food, such as fresh vegetables in local markets. A high correlation was observed between mcr-1 and crAssphage, which is a human-specific fecal marker. As most wastewater is not treated in Vietnam, the dissemination of mcr-1 into the aqueous environment and food chain is likely attributable to the recycling of this untreated wastewater. We further found that in 26 urban rivers in Japan, ARGs were extracted from the SmartChip analysis that showed a positive correlation with class 1 integron-integrase gene (int1I). This result demonstrates that the abundance of intI1 can be used to comprehensively assess the levels of some ARGs in the water environment. Extensive water-monitoring data should be accumulated to determine the regulatory levels of ARGs in water in the framework of One Health.

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Bio

Dr. Kasuga obtained his Ph.D. from the University of Tokyo in 2005 and continued working there from 2006. In September 2018, he was dispatched to VNU-Vietnam Japan University in Hanoi, Vietnam. His research interests include antibiotic resistance in water environment, biological water treatment, and drinking water microbiome.

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